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. 2017 Dec 5;7:16945. doi: 10.1038/s41598-017-16095-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Formation of LCVs in CLas and CLso infected gut cells. CLSM images of D. citri guts stained with ER Tracker (green) together with CLas immunolocalization using a specific antibody against the outermembrane protein A (OmpA) of Liberibacter (red). Nuclei were stained with DAPI (blue). (A) CLas infected D. citri gut showing formation of ER intracellular bodies and immunolocalization of CLas inside ER bodies. (B) CLas immunolocalization inside ER associated LCVs and nuclei defregmantation. (C) ER staining in guts from CLso-infected B. trigonica adults show specific immulolocalization of CLso but no association with the ER or LCV formation.