FIG 6.

The BtsSR/YpdAB network promotes homogeneous protein overproduction in all cells. Wild-type (WT) or btsSR ypdAB mutant cells harboring the overproduction vector pBAD24-gfp were grown in LB medium. Samples were taken before and after the addition of the inducer arabinose (Ara) (0.2% [wt/vol]). The cells were analyzed by fluorescence microscopy and flow cytometry. Distributions of fluorescent cell counts and representative views of WT cells before and after addition of arabinose are shown in panels A and B, while the corresponding data for the btsSR ypdAB mutant are depicted in panels C and D. About 2,000 events were recorded for each plot. Cell counts represent the numbers of cells, and fluorescence intensity is expressed in arbitrary units (AU). Scale bar, 2 μm. Experiments were performed independently three times.