FIG 2.

Effects of PCF or BCF on A. fumigatus strain 10AF preformed biofilm growth and metabolism. A. fumigatus strain 10AF conidia in agar were distributed into 96-well cell culture plates. Plates were incubated at 37°C for 24 h to allow hyphal growth and then loaded with wild-type or mutant PA14 PCF (A) or wild-type or mutant PA14 BCF (B) and incubated at 37°C for 24 h. A. fumigatus strain 10AF metabolism was evaluated by XTT assay. Metabolism in the presence of RPMI medium alone was regarded as 100% A. fumigatus strain 10AF metabolic activity. The data shown are the mean ± SD of four independent experiments (with duplicates of each group studied in each experiment). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (mutant [no. 1 to 24] versus wild type [no. 25]). Horizontal asterisks, RPMI medium versus wild-type PA14. Bars: 1, pvdD pchE mutant; 2, pqsE mutant; 3, mvfR mutant; 4, pqsA mutant; 5, pqsH mutant; 6, lasR rhlR mutant; 7, lasR mutant; 8, rsmA mutant; 9, pqsA pqsH not polar mutant; 10, pvdD mutant; 11, rhlR mutant; 12, ΔHSI-I ΔHSI-II mutant; 13, pvcA mutant; 14, rhlA mutant; 15, phzC1 phzC2 mutant; 16, pchE mutant; 17, exoU mutant; 18, rsmY rsmZ mutant; 19, ΔHSI-II ΔHSI-III mutant; 20, ΔHSI-I ΔHSI-III mutant; 21, pqsA pqsH polar mutant; 22, chiC mutant; 23, lecA mutant; 24, hcnA mutant.