TABLE 2.
Ranking of PA14 mutant LC, PCF, and BCF activities against A. fumigatus strain 10AF forming biofilma
| Rank | Mutation(s) ranked on basis of: |
||
|---|---|---|---|
| Inhibition zone size, agar-LC | XTT conversion, agar-PCF | XTT conversion, agar-BCF | |
| 1 | pvdD pchE | pvdD pchE | pvdD pchE |
| 2 | lasR | pvdD | pvdD |
| 3 | lasR rhlR | lasR | lasR rhlR |
| 4 | pqsA pqsH polar | lasR rhlR | lasR |
| 5 | pvcA | rhlR | |
| 6 | phzC1 phzC2 | pvcA | |
| 7 | rhlA | pqsA pqsH polar | |
| 8 | ΔHSI-I ΔHSI-II | pqsA | |
| 9 | pchE | pqsH | |
| 10 | rhlR | ||
| 11 | rsmY rsmZ | ||
| 12 | pvdD | ||
a
All assays started from Aspergillus conidia. Ranking from 1 to n represents highest to lowest loss of antifungal activity. Ranking was performed as described in Materials and Methods (Fig. 1). Only isolates statistically significantly different from the parent strain are listed.