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. 2017 Dec 5;7:16928. doi: 10.1038/s41598-017-17155-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of constitutively activate WalR-R204C. (A) Growth of strain 168, GYQ215 (amyE::Pxyl-sepF) and strain GYQ159 (amyE::Pxyl-sepF aprE::Pspac-walR-R204C) in medium with 1% xylose to induce SepF. In case of strain GYQ159, 1 mM IPTG was added to induce the constitutively active WalR variant R204C (walR*). After 4 h, the culture was diluted. (B) Microscopy images of GYQ159 at t = 6 h. Septa are indicated by arrows. Cells were stained with the membrane dye FM5-95. Scale bar is 5 µm. (C) Cell length measurement of 168, GYQ215 (−WalR*) and GYQ159 (+WalR*). Samples were taken at t = 3 h.