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. 2017 Dec 5;7:16928. doi: 10.1038/s41598-017-17155-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SepF overexpression and pbpB depletion delocalize WalK. (A) Microscopic images of strain GYQ571 (amyE::Pxyl-sepF ftsZ-gfp aprE::Pspac-mcherry-walK) expressing both FtsZ-GFP and mCherry-WalK, grown without or with 1% xylose to induce SepF overexpression. Additional images shown in Fig. S7. (B) Septum-localization of WalK is abolished in cells depted for pbpB (strain GYQ174, pbpB::Pspac-pbpB amyE::Pxyl-gfp-walK). Membranes were stained with FM5-95. Scale bar is 5 µm.