FIG 5.

Relative locations of the epitopes recognized by 30 different RTA-specific V_HHs. Thirty RTA-specific V_HHs (left column) were subjected to cross-competition sandwich ELISA with a panel of nine murine MAbs (top row) representing the four different epitope clusters on RTA. Shown here is a binary representation of the actual sandwich ELISA (Fig. S6) in which individual cells are colored red for >50% competition (V_HH versus MAb) and green for <50% competition. V_HH clonal families are boxed (left column). V_HHs with the same competition profile were grouped into bins (right column). For reference, V_HH binding affinities and TNA (from Table 2) are shown in the far right columns.