FIG 7.

Relative locations of the epitopes recognized by holotoxin- or RTB-specific V_HHs. Twenty holotoxin (*)- or RTB-specific V_HHs (left column) were subjected to cross-competition sandwich ELISA with a panel of nine murine MAbs and ASF (top row). Shown here is a binary representation of the actual sandwich ELISA (Fig. S8) in which individual cells are colored red for >50% competition (V_HH versus MAb) and green for <50% competition. V_HHs with the same competition profile were grouped together into bins (right column). For reference, V_HH binding affinity data and TNA data (from Table 2) are shown in the far right columns.