FIG 2.

Immunization with GI-19007 elicits an antigen-specific IFN-γ response in mice. C57BL/6 mice were immunized intradermally with 2.5 YU per flank of GI-19007 or empty yeast vector (YVEC) on days 0 and 56. Ten days later, spleens were removed and stimulated in a 24-h ELISpot assay with 6 μg/ml of pooled recombinant antigens or the same concentration of irrelevant antigen. The ratio of IFN-γ ELISpot counts between the two are expressed as a stimulation index.