FIG 1.

Relative levels of backbone flexibility of RiVax, as determined by HX-MS. RiVax was subjected to HX-MS analysis, as described in Materials and Methods. The extent of hydrogen exchange (HX) at 13 s for each RiVax peptide is shown in panel A. The peptides are color coded according to the results of k-means categorization as follows: rigid, blue; intermediate, gray; flexible, yellow. The peptide number indicates a sequential arrangement from the N terminus to the C terminus. The peptide assignments are provided in Table S1 in the supplemental material. (B) The flexibility categories mapped onto the structure of RiVax.