FIG 2.
Catalase activities measured by the Amplex Red-based assay. PBS control (filled triangle), 11168 (filled square), CMT (filled circle), 11168 OXR mutant 11168 perRA338Del (open square), and CMT OXR mutant CMT perRC143A (open circle) were used in the assay. Amplex Red reagent was added to the reaction mixtures at 5-min intervals, and fluorescence signals from the oxidation indicator product resorufin were measured in triplicate wells. The experiments were carried out at least three times, and a representative result is shown.