TABLE 1.
Antifungal activity and culture media for the isolation of culturable endophytic actinobacteria isolated from the root system of grafted grapevines
| Isolate | I indexa |
Detection of antifungal activityb |
Isolation medium | 16S rRNA sequence accession no. | ||
|---|---|---|---|---|---|---|
| D. seriata | D. macrodidyma | P. chlamydospora | P. minimum | |||
| Streptomyces sp. VV/E1 | 53.47 | 33.91 | + | + | SC | KY978646 |
| Streptomyces sp. VV/E2 | 61.17 | 59.41 | + | + | ISP2 | KY978647 |
| Saccharopolyspora sp. VV/E3 | 31.97 | 35.87 | − | − | SC | KY978648 |
| Streptomyces sp. VV/E4 | 46.95 | 46.62 | − | + | SC | KY978649 |
| Streptomyces sp. VV/E5 | 52.50 | 47.15 | + | + | SC | KY978650 |
| Streptomyces sp. VV/E6 | 49.57 | 33.86 | ND | ND | DPA | KY978651 |
| Saccharopolyspora pathumthaniensis VV/E7 | 36.81 | — | ND | ND | ISP2 | KY978652 |
| Streptomyces sp. VV/E8 | 33.42 | — | ND | ND | DPA | KY978653 |
| Streptomyces sp. VV/E9 | — | 40.24 | ND | ND | DPA | KY978654 |
| Saccharopolyspora sp. VV/E10 | 29.97 | — | ND | ND | ISP2 | KY978655 |
| Streptomyces sp. VV/E11 | — | 40.24 | ND | ND | SAA | KY978656 |
| Streptomyces atrovirens VV/E12 | — | — | ND | ND | ISP2 | KY978657 |
| Streptomyces sp. VV/E13 | — | — | ND | ND | ISP2 | KY978658 |
| Streptomyces sp. VV/E14 | — | — | ND | ND | SAA | KY978659 |
| Streptomyces sp. VV/E15 | — | — | ND | ND | SC | KY978660 |
| Streptomyces sp. VV/E16 | — | — | ND | ND | SC | KY978661 |
| Streptomyces sp. VV/E17 | — | — | ND | ND | ISP2 | KY978662 |
| Streptomyces sp. VV/E18 | — | — | ND | ND | SAA | KY978663 |
| Micromonospora sp. VV/E19 | — | — | ND | ND | ISP2 | KY978664 |
| Micromonospora sp. VV/E20 | — | — | ND | ND | ISP2 | KY978665 |
| Micromonospora sp. VV/E21 | — | — | ND | ND | SC | KY978666 |
| Streptomyces sp. VV/E22 | — | — | ND | ND | SC | KY978667 |
| Streptomyces sp. VV/E23 | — | — | ND | ND | DPA | KY978668 |
| Streptomyces sp. VV/E24 | — | — | ND | ND | SC | KY978669 |
| Streptomyces sp. VV/E25 | — | — | ND | ND | SC | KY978670 |
| Streptomyces sp. VV/E26 | — | — | ND | ND | DPA | KY978671 |
| Micromonospora palomenae VV/E27 | — | — | ND | ND | ISP2 | KY978672 |
| S. pathumthaniensis VV/E28 | — | — | ND | ND | SC | KY978673 |
| Streptomyces sp. VV/E29 | — | — | ND | ND | ISP2 | KY978674 |
| Streptomyces sp. VV/E30 | — | — | ND | ND | SC | KY978675 |
| S. pathumthaniensis VV/E31 | — | — | ND | ND | ISP2 | KY978676 |
| Streptomyces sp. VV/32 | — | — | ND | ND | SC | KY978677 |
| S. pathumthaniensis VV/E33 | — | — | ND | ND | ISP2 | KY978678 |
| Rathayibacter caricis VV/E34 | — | — | ND | ND | ISP2 | KY978679 |
| Nonomuraea kuesteri VV/E35 | — | — | ND | ND | ISP2 | KY978680 |
| S. pathumthaniensis VV/E36 | — | — | ND | ND | SC | KY978681 |
| N. kuesteri VV/E37 | — | — | ND | ND | SC | KY978682 |
| S. pathumthaniensis VV/E38 | — | — | ND | ND | ISP2 | KY978683 |
| Micromonospora sp. VV/E39 | — | — | ND | ND | ISP2 | KY978684 |
| Micromonospora sp. VV/E40 | — | — | ND | ND | SC | KY978685 |
| Micromonospora sp. VV/E41 | — | — | ND | ND | ISP2 | KY978686 |
| Micromonospora sp. VV/E42 | — | — | ND | ND | ISP2 | KY978687 |
| Micromonospora sp. VV/E43 | — | — | ND | ND | ISP2 | KY978688 |
| Streptomyces sp. VV/E44 | — | — | ND | ND | SAA | KY978689 |
| Micromonospora sp. VV/E45 | — | — | ND | ND | SC | KY978690 |
| Micromonospora sp. VV/E46 | — | — | ND | ND | ISP2 | KY978691 |
| Micromonospora sp. VV/E47 | — | — | ND | ND | ISP2 | KY978692 |
| Micromonospora sp. VV/E48 | — | — | ND | ND | SC | KY978693 |
| S. pathumthaniensis VV/E49 | — | — | ND | ND | SC | KY978694 |
| Micromonospora sp. VV/E50 | — | — | ND | ND | ISP2 | KY978695 |
| Nonomuraea sp. VV/E51 | — | — | ND | ND | SC | KY978696 |
| S. pathumthaniensis VV/E52 | — | — | ND | ND | ISP2 | KY978697 |
| Streptomyces sp. VV/E53 | — | — | ND | ND | DPA | KY978698 |
| Nonomuraea sp. VV/E54 | — | — | ND | ND | ISP2 | KY978699 |
| Kribbella sp. VV/E55 | — | — | ND | ND | SAA | KY978700 |
| Corynebacterium sp. VV/E56 | — | — | ND | ND | ISP2 | KY978701 |
| Micromonospora sp. VV/E57 | — | — | ND | ND | ISP2 | KY978702 |
| Streptomyces sp. VV/E58 | — | — | ND | ND | SAA | KY978703 |
a
Inhibition index (I index) values shown are the average of three independent experiments. —, species not showing antifungal activity against D. seriata or D. macrodidyma.
b
ND, not determined.