FIG 7.

Inhibition of proper biofilm formation by excess LytF. Biofilms of S. sanguinis SK36, ΔlytF, and lytF-c strains were grown in CDM for 18 h, washed, and stained with 0.01% safranin. Biofilm formation was determined by measuring the absorbance of air-dried biofilms at 490 nm. The lytF-c biofilms showed a significant decrease in absorbance after safranin staining, indicating that biofilm formation was inhibited in this strain (****, P < 0.0001). Data represent averages and standard deviations from quadruplicate samples in three separate plate experiments.