TABLE 2.

Primers used

Primer name	Oligonucleotide sequence (5′ to 3′)a	Purposeb	Reference
Strep. 16S rRNA-RT-F	AAGCAACGCGAAGAACCTTA	qRT-PCR	4
Strep. 16S rRNA-RT-R	GTCTCGCTAGAGTGCCCAAC	qRT-PCR	4
Ss lytF F	CTCCTGCGTCATACCACTGA	qRT-PCR
Ss lytF R	CAGGAGGTACAGGACGCATT	qRT-PCR
Ss comC RTF	TGAAAATCTATTCTTTTCAAATTGC	qRT-PCR
Ss comC RTR	CCATGGATTTGGAACACCTC	qRT-PCR
Ss comD RTF	GGAGATTCAGCTTTAAGGAGTGTC	qRT-PCR	31
Ss comD RTR	ACAACTTGATTGGAAGGCGTTC	qRT-PCR	31
Ss comE RTF	TTGGAATTGACATCCAGGTTC	qRT-PCR
Ss comE RTR	TGTTTCCTCCCCCTTAATGTC	qRT-PCR
Ss comX RTF	CAAGAAAGCCAAAAGCGAAA	qRT-PCR
Ss comX RTR	TCGCTTCTCTGAAGGCAACT	qRT-PCR
Ss comYA RTF	TGATTAGGCAGGCTCGGCAAGAAG	qRT-PCR	31
Ss comYA RTR	AAGCGGCGTTCATCACCAATTCTC	qRT-PCR	31
Ss purD RTF	GCGTGGCTTCTGTCAAGATT	qRT-PCR
Ss purD RTR	CCAGCTTCTGCGATTTCTTC	qRT-PCR
Ss purH RTF	GAGCTGGAGTGGTCCAAAGA	qRT-PCR
Ss purH RTR	TCCCTGCTTTTCGATGTAGG	qRT-PCR
Ss lytF US-F	TGGAATGTTCGGAAATTCCA	lytF KO
Ss lytF US-R	ATCAAACAAATTTTGGGCCCGGGATATTTCTTCATAAAACTC	lytF KO
Ss lytF DS-F	ATTCTATGAGTCGCTGCCGACTCCTAAATAAGTCGCTTTCGC	lytF KO
Ss lytF DS-R	CGAGTGGCTTTGGAATTTGC	lytF KO
Erm-L	CCGGGCCCAAAATTTGTTTGAT	Erythromycin cassette
Erm-R	AGTCGGCAGCGACTCATAGAAT	Erythromycin cassette
SS lytF-c_L	AGTGAATTCGAGCTCGGTACCCGGACCAAGCTAACACATCACGTA	lytF complementation
SS lytF-c_R	AACAGCTATGACCATGATTACGCCGAGCAGTCTGCTCAAAGTAG	lytF complementation
pDL278_1L	TCCCCGGGTACCGAGCTCGAATTCACT	Concatenation
pDL278_1R	CTTGGCGTAATCATGGTCATAGCTGTT	Concatenation
5′ SS lytF gs-F	ATTATCCGGAGCTGTCCTAGGT	5′ RACE
5′ SS lytF gs inner-R	AAGCTTGATAAGGTTCTTGAGCTTGAGAACG	5′ RACE
5′ SS lytF gs outer-R	AGCGCTCTCCTATCCGTATGTAAG	5′ RACE

^aOligonucleotides were created for this study unless otherwise specified.

^bKO, knockout.