TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or descriptiona | Reference or source |
|---|---|---|
| Strains (P. putida) | ||
| B6-2 | Wild type (DSM 28064) | 7, 8 |
| B6-2ΔsrpABC | srpABC-knockout mutant of strain B6-2 | This study |
| B6-2ΔttgABC | ttgABC-knockout mutant of strain B6-2 | This study |
| B6-2ΔsrpS | srpS-knockout mutant of strain B6-2 | This study |
| B6-2ΔttgABCΔsrpS | ttgABC- and srpS-null mutant of B6-2 | This study |
| B6-2ΔttgR | ttgR-null mutant of B6-2 | This study |
| B6-2ΔbphC | bphC-deficient mutant of strain B6-2 | This study |
| B6-2ΔbphD | bphD-deficient mutant of strain B6-2 | This study |
| B6-2ΔbphCΔsrpABC | srpABC-knockout and bphC-deficient mutant of strain B6-2 | This study |
| B6-2ΔbphA(pMEG) | bphA-deficient mutant containing plasmid pMEG | This study |
| B6-2ΔbphC(pMEG) | bphC-deficient mutant containing plasmid pMEG | This study |
| B6-2ΔbphD(pMEG) | bphD-deficient mutant containing plasmid pMEG | This study |
| B6-2(pMEG) | B6-2 containing plasmid pMEG | This study |
| B6-2(pMEA) | B6-2 containing plasmid pMEA | This study |
| B6-2ΔsrpS(pMEG) | B6-2ΔsrpS containing plasmid pMEG | This study |
| B6-2ΔttgR(pMEA) | B6-2ΔttgR containing plasmid pMEA | This study |
| Plasmids | ||
| pK18mobsacB | Mobilizable vector, mob+, sacB, Kmr | 35 |
| pK18G | pK18mobsacB::ΔsrpABC | This study |
| pK18A | pK18mobsacB::ΔttgABC | This study |
| pK18S | pK18mobsacB::ΔsrpS | This study |
| pK18R | pK18mobsacB::ΔttgR | This study |
| pK18bphA′ | pK18mobsacB::bphA′ | This study |
| pK18bphC′ | pK18mobsacB::bphC′ | This study |
| pK18bphD′ | pK18mobsacB::bphD′ | This study |
| pME6015 | promoterless lacZ vector, Tcr | This study |
| pMEG | Tcr, srpA promoter cloned in pME6015 | This study |
| pMEA | Tcr, ttgA promoter cloned in pME6015 | This study |
a
Kmr, resistance to kanamycin; Tcr, resistance to tetracycline.