TABLE 3.

Oligonucleotides used in this study

Primer	Sequence (5′→3′)a	Purpose
G-A1	GCATGAATTCTCCCCGCGTGCATTACGGGT	Knock out srpABC
G-A2	TGGGCTTGGTTCACCCATGCGGCAAGCGCATCGTC
G-B1	TGCGCTTGCCGCATGGGTGAACCAAGCCCAGGCCC
G-B2	GCATAAGCTTGGCTCGCAGCTTTCCTGCGT
A-A1	GCATGAATTCACACGCTCACTGGCCGAACG	Knock out ttgABC
A-A2	GCCTTGAGCAGGTGCTGAACCGAGGCGGCGTTGTC
A-B1	CGCCGCCTCGGTTCAGCACCTGCTCAAGGCTGCCA
A-B2	GCATAAGCTTGCGTTCGGCCAAGCGGTAGT
G-F	CTCTGAAGTTTTCCGAGGTG	Identify the ΔsrpABC mutant
G-R	AGCCGAGGCTTCTGTGGTAG
A-F	GTCGGCGTCGTGACCATCCA	Identify the ΔttgABC mutant
A-R	CACCGATGCCTGCTGGTTGG
S-A1	GCATGAATTCGGGGATCGTATCTGTCTCAC	Knock out srpS
S-A2	AGCTGGATCCTCTGGCGATGACCTGGATGC
S-B1	AGCTGGATCCCAGCATTACCTGACGAAACCCTA
S-B2	GCATAAGCTTCATCTTATCTAGGGAGCTTTCTTCGA
S-F	GCATGAATTCCGCTCCACCGTTCAGAGAAT	Identify the ΔsrpS mutant
S-R	GCATAAGCTTGTTTGACAAGCGCCTTTCGT
R-A1	GCATGAATTCCTGCTGCAAGGGGGTTTCGAATTG	Knock out ttgR
R-A2	AGCTGGATCCATATGCTGCGCTTGAGCCCG
R-B1	AGCTGGATCCCGCGCCAAATGGTCATGGGTCT
R-B2	GCATAAGCTTAGCTTATCCGAGAGGCCCCG
R-F	CAGCCCGGTGTCGACCCATT	Identify the ΔttgR mutant
R-R	CTGGTGCTCGATGCCCGAAC
PsrpA-F	GCATGAATTCCCCGGCCTTGCCAATATTTT	Clone the promoter of srpA
PsrpA-R	GCATCTGCAGTAATGCACGCGGGGATCGTA
PttgA-F	GCATGAATTCGGGTTTCCTGGGCTTCTTCTTTGGT	Clone the promoter of ttgA
PttgA-R	GCATCTGCAGTGGCTTGAATTGCATGAGGAT
q A-F	GCAGTGAGGTCAAGGAAGG	RT-qPCR for ttgA
q A-F	CGTGTAGCCAGCAGGTTG
q G-F	CGTCACTCAGCCAATCAC	RT-qPCR for srpA
q G-F	GATAAGCACTTCCGTCATCC
16S-F	ACGCTAATACCGCATACG	RT-qPCR for 16S rRNA
16S-R	CATCCTCTCAGACCAGTTAC
pK-bphA-F	GCATGAATTCGGTTTTTGGTCGCTCTTGGC	Construct a bphA interruption
pK-bphA-R	GCATAAGCTTGAAGACCAGGCCCTTGTAGGTG
pK-bphC-F	GCATGAATTCGACGGTTCCTGTGTACTTCCT	Construct a bphC interruption
pK-bphC-R	AGCTGGATCCCTCGGACTGTCGTGCCTCA
pK-bphD-F	GCATGAATTCTAACGGCGAAACCGTCATCA	Construct a bphD interruption
pK-bphD-R	AGCTGGATCCAGCAGCTTGATGCCTTCCAT
pK269	GCTTCCCAACCTTACCAGA	Identify the single exchange mutant

^aEngineered restriction sites are underlined.