TABLE 2.
C. bescii strains used and constructed for this study
| Strain | Parent | Transforming plasmid | Genotype | Reference |
|---|---|---|---|---|
| DSM 6725 | Wild type | 63 | ||
| JWCB005 | DSM 6725 | pDCW88 | ΔpyrFA | 64 |
| RKCB120 | JWCB005 | pJMC021 | ΔpyrFA ΔAthe_1867::PslpCbhtk | This study |
| RKCB121 | JWCB005 | pJMC021 | ΔpyrFA Δ(Athe_1867-Athe_1857)::PslpCbhtk | This study |
| RKCB123 | JWCB005 | pJMC021 | ΔpyrFA Δ(Athe_1867-Athe_1865′)::PslpCbhtk | This study |
| RKCB124 | JWCB005 | pJMC033 | ΔpyrFA ΔAthe_1860::PslpCbhtk | This study |
| RKCB125 | JWCB005 | pJMC034 | ΔpyrFA ΔAthe_1857::PslpCbhtk | This study |
| RKCB127 | JWCB005 | pJMC034 | ΔpyrFA Δ(Athe_1857-Athe_1865′)::PslpCbhtk | This study |
| RKCB130 | JWCB005 | pJMC069 | ΔpyrFA Δ(Athe_1867-Athe_1859)::PslpCbhtk | This study |
| RKCB132 | JWCB005 | pJMC070 | ΔpyrFA ΔAthe_1859::PslpCbhtk | This study |