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. 2017 Dec 5;7:16923. doi: 10.1038/s41598-017-16916-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HPRT1 mRNA expression levels normalized with different reference genes in human placenta. RPS18 has significantly different Cq values between sexes. IPO & TBP were selected using NormFinder software. TBP & YWHAZ were selected using GeNorm software. RPL30 and RPL30 & GAPDH were selected using the ΔCq form the placental sex multiplied by GeNorm M value. *p ≤ 0.05; **p ≤ 0.01 n = 10 per placental sex.