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. 2017 Dec 5;7:17011. doi: 10.1038/s41598-017-17274-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of PfCSP@PbCSP and PfCSP@PbUIS4 parasites with PfCSP vaccinated serum samples. (a) In 3 separate experiments, serum from BALB/c mice vaccinated with 10⁷ iu ChAd63.PfCSP 10⁶ MVA.PfCSP (filled circle), 10⁸ iu ChAd63.PfCSP 10⁷ pfu MVA.PfCSP (closed squares) or 3 μg R21 in Matrix M (filled triangles) were tested for the ability to inhibit the invasion of Huh7 cells with PfCSP@PbCSP sporozoites. In each experiment, the grey bars denote the level of inhibition observed with naïve serum samples. Data in each graph was analysed with a two-way ANOVA and post-hoc Sidaks multiple comparison, asterisk denote the level of significance comparing PfCSP samples against naïve serum samples, *p < 0.05 ****p < 0.0001. The percentage of inhibition observed with 2% serum concentration was compared to antibody titres measured by ELISA (right) and analysed with a two-tailed Spearmans correlation. (b) In 3 separate experiments, serum from BALB/c mice vaccinated with 10⁷ iu ChAd63.CSP 10⁶ MVA.CSP (filled circle), 10⁸ iu ChAd63.CSP 10⁷ pfu MVA.CSP (closed squares) or 3 μg R21 in Matrix M (filled triangles) were tested for the ability to inhibit the invasion of Huh7 cells with PfCSP@PbUIS4 sporozoites. In each experiment, the grey bars denote the level of inhibition observed with naïve serum samples. Data in each graph was analysed with a two-way ANOVA and post-hoc Sidaks multiple comparison, asterisk denote the level of significance comparing PfCSP samples against naïve serum samples, *p < 0.05 ****p < 0.0001. The percentage of inhibition observed with 2% serum dilutions was compared to antibody titres measured by ELISA (right) and analysed with a two-tailed Spearmans correlation (p = 0.0318). (c) Serum samples from human volunteers vaccinated with a ChAd63.PfCSP followed by MVA.PfCSP were tested for the ability to inhibit the invasion of Huh7 cells with PfCSP@PbCSP sporozoites. Pre-vaccination (circle) and 2 to 3 week post MVA boost (square) boost serum samples were tested at 10% and 20% serum concentrations (left) and the percentage of inhibition observed with 10% serum concentrations was compared to the level of PfCSP specific antibodies measured by ELISA (right) with data analysed with a non-parametric Spearmans test, but no significant correlation was observed. (d) Serum samples from human volunteers vaccinated with a ChAd63.PfCSP followed by MVA.PfCSP were tested for the ability to inhibit the invasion of Huh7 cells with PfCSP@PbUIS4 sporozoites. Pre-vaccination (circle) and 2 to 3 week post MVA boost (square) boost serum samples were tested at 2% and 10% serum concentrations (left) and the percentage of inhibition observed with 10% serum concentrations was compared to the level of PfCSP specific antibodies measured by ELISA (right) with data analysed with a non-parametric Spearmens test, but no significant correlation was observed.