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. 2017 Dec 5;7:16956. doi: 10.1038/s41598-017-17034-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative micrographs of two PD-L1 IHC assays for TCs or ICs. (A) 22C3-IHC (80%) and SP142-IHC (70%) assays indicating strong PD-L1-positivity in TCs; (B) 22C3-IHC (10%) and SP142-IHC (10%) assays indicating strong PD-L1-positivity in ICs; (C) 22C3-IHC (30%) and SP142- IHC (5%) assays indicating weak PD-L1-positivity in TCs; and (D) 22C3-IHC (10%) assay indicating strong PD-L1-positivity in ICs, whereas SP142-IHC assay indicative negative staining in ICs.