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. 2017 Dec 5;7:16994. doi: 10.1038/s41598-017-17259-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of Mia40 and GSSG in the oxidative folding reaction of hCox17. (A) Reduced hCox17 was allowed to refold in the presence of Mia40 (molar ratio 1:2) and the reaction analyzed by RP-HPLC. (B) The normalized peak area of reduced (red triangles), intermediate (blue squares) and native (green circles) species are represented as a function of time in the presence (left) and absence (right) of Mia40. The fitting to a U-I-N transition is displayed in solid lines. (C) hCox17 folding intermediate disappearance as a function of time in the presence of Mia40 (open circles) and in stop and go assays (solid squares). A dashed line at 50% is also represented to identify the half-life of the specie. (D) RP-HPLC chromatograms of hCox17 folding reaction in the presence of 1 mM GSSG. Red boxes highlight off-pathway intermediates.