Extended Data Figure 5. The novel D2SP improves expression and specificity over previously published promoters.
a, Expression of eYFP under the novel D2SP. Red shows D2R immunostaining (Methods). Scale bar, 100 μm. b, Expression of eYFP under a D2R promoter based on previously published constructs (D2RE), which included the first exon of the D2 receptor gene30,31. Image taken with settings matched to those used for the D2SP image in a. Scale bar, 100 μm. c, Images are of the same field of view as in b but taken with settings optimized to see the (otherwise dim) eYFP expression. Scale bar, 100 μm. d, Specificity of expression under the D2SP improved from 90.5% to 98.2% under the previously described promoter. Penetrance of expression under the DR2 promoter improved from 69% to 86.8% under the previously described promoter. e, Full sequence of D2SP.