Figure 2.

Knockdown of ATP6V0C has no effect on levels of representative viral immediate early or late transcripts. Fibroblast cells were transfected with ATP6V0C siRNA or a control siRNA and infected 48 hpi. Total RNA was harvested at indicated time points. Levels (a) of IE86, (b) UL83 and (c) UL75 were determine by qRT-PCR using specific primer probe assays (n = 2). (d) Cell-associated viral protein levels were determined by western blot analysis at the indicated time points post-infection from control cells (siNeg) or cells knocked down for ATP6V0C (siA6).