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. 2017 Nov 1;7(11):160298. doi: 10.1098/rsob.160298

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — ATP6V0C is not required for generation of viral capsids in the nucleus. To directly analyse the effects of ATP6V0C knockdown on intracellular viral particle formation, infected cells were imaged by transmission electron microscopy at 120 hpi. Fibroblast cells were transfected with (a) ATP6V0C siRNA or (b) negative control siRNA and infected with HCMV (AD169). (c) Enlarged image from (b) showing three capsid types: A—empty capsid, B—capsid with scaffold, C—DNA containing capsid. (d) Manual counting of images revealed no significant difference in nuclear capsid formation following ATP6V0C knockdown. Nine individual frames from three independent cells were counted for each condition. A total of 508 capsid particles were counted for control cells and 516 particles counted for ATP6V0C knockdown cells.