Figure 4.

ATP6V0C is required for HCMV virion assembly compartment biogenesis. Fibroblast cells were transfected with ATP6V0C siRNA or negative control siRNA and at 72 h post-transfection were infected with AD169. At 144 hpi cells were fixed, permeabilised and stained for early endosomes or trans-Golgi vacuoles, (EEA1:green or TGN46:green), viral tegument protein (pp28:red) and nuclei (DAPI:blue). Images were acquired on a Zeiss LSM710 confocal microscope. Images presented here are maximum-intensity projections compiled from multiple 0.33 µm slices through the z-axis.