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Disruption of V-ATPase complex results in loss of VAC formation. (a) Immunofluorescence microscopy in AD169 infected fibroblast cells transfected with ATP6V1H siRNA and stained for early endosomes (EEA1/green), viral tegument protein (pp28/red) and nuclei (DAPI/blue). Images represent single slices through the z-axis. (b) Pearson's R-value for colocalization of TGN46 or EEA1 and pp28 in ATP6V1H or negative control siRNA transfected fibroblast cells (n = 20). **p-value < 0.01.
