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. 2017 Nov 8;7(11):170111. doi: 10.1098/rsob.170111

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Heat map for up- and downregulated genes in OKF6HPV-16E6E7 cells. Total RNAs extracted from stably transfected (pLXSNHPV16E6E7) and empty vector transfected (pLXSN) OKF6–Tert2 cells were separately labelled by reverse transcription with Cyanine-5 and Cyanine-3 dCTPs dyes and co-hybridized to HEEBO-48 K microarrays. Raw data were collected as GPR files and normalized by print-tip Lowess. A LIMMA test was applied to determine differentially expressed genes using an adj-p value < 0.05 and a log2 fold-change > (±) 1.0 as cut-off. The 30 top-ranked up- and downregulated transcripts are shown.