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. 2017 Nov 8;7(11):170111. doi: 10.1098/rsob.170111

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 5. — PIR expression is associated with increased EMT and migration in Ca Ski cells. (a–d) Ca Ski cells were transiently transfected with an siRNA for PIR silencing, and the mRNA levels of (a) E-cadherin, (b) SLUG, (c) ZEB and (d) Snail were measured by RT–qPCR. (e) (i) Transwell assay for Ca Ski migration after transfection with an siRNA for PIR silencing. (ii) Cell count after 7 h of migration. Results are representative of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.