Table 1.
Subunits of the human anaphase promoting complex/cyclosome (APC/C). Alternative S. cerevisiae subunits in parenthesis.
| subunit | length (aa) | stoichiometry | location | domain/Region 1 | domain/Region 2 | domain/Region 3 | phosphorylation sites (from ref. [60]) |
|---|---|---|---|---|---|---|---|
| Apc1 | 1944 | 1 | scaffolding module platform |
WD40 domain (1–612) | mid-N (613–986) mid-C (1617–1944) |
PC domain (1013–1616) | 60,65,202,233,286,291,297,298,299,309,313,316,317,341,343,351,355,362,364,372,373,377,386,389,394,416,501,518,520,522,524,530,536,537, 542,547,555,563,564,569,576,582,600,686,688,699,701,703,731,916,920,921,922,1001,1347,1349 |
| Apc2 | 822 | 1 | catalytic module | NTD (1–432) cullin repeats |
CTD (433–822) including WHB domain |
— | 205,218,314,466,470,474,532,534,732,736,738,742 |
| Apc3A Apc3B (Cdc27) |
824 | 2 | scaffolding module TPR lobe |
TPR dimer interface TPR motifs 1–7 (1–535) |
TPR superhelix TPR motifs 8–14 (536–824) |
— | 183,185,186,192,194,200,203,205,209,219,220,222,225,228,230,231,233,237,241,244,251,252,255,264,267,276,279,281,289,291,302,304,312, 313,327,329,331,334,336,343,349,351,352,356,357,358,364,366,368,369,383,384,386,387,388,389,419,426,430,434,435,438,443,444,446, 761,800,803,806,807,809,814,821 |
| Apc4 | 808 | 1 | scaffolding module platform | WD40 domain/4HBD | — | — | 199,469,488,757,758,777,779 |
| Apc5 | 755 | 1 | scaffolding module platform |
NTD (1–169) | TPR superhelix TPR motifs 1–13 (206–755) |
— | 15,130,178,179,195,221,228,232,674 |
| Apc6A Apc6B (Cdc16) |
620 | 2 | scaffolding module TPR lobe |
TPR dimer interface TPR motifs 1–7 (1–261) |
TPR superhelix TPR motifs 8–14 (262–620) |
— | 112,559,573,577,580,584,585,592,599,607,614 |
| Apc7A Apc7B |
599 | 2 | scaffolding module TPR lobe |
TPR dimer interface TPR motifs 1–3 (21–166) |
TPR dimer interface TPR motifs 4–7 (167–359) |
TPR superhelix TPR motifs 8–14 (360–599) |
119,120,123,125,126,573,582,584 |
| Apc8A Apc8B (Cdc23) |
597 | 2 | scaffolding module TPR lobe |
TPR dimer interface TPR motifs 1–7 (1–287) |
TPR superhelix TPR motifs 8–14 (288–597) |
— | 562,565,582,584,588,593,596 |
| Apc10 | 185 | 1 | degron recognition module | Doc homology (2–162) | IR tail (163–185) | — | — |
| Apc11 | 84 | 1 | catalytic module | β-strand (1–18) | RING domain (21–84) | — | — |
| Apc12A Apc12B (Cdc26) |
85 | 2 | scaffolding module TPR lobe |
N-term (1–25) Extended chain, short α-helix | — | — | 42,51,52,56,78 |
| Apc13 (Swm1) |
74 | 1 | scaffolding module TPR lobe |
extended chain (1–67) | — | — | |
| Apc15 (Mnd2) |
121 | 1 | scaffolding module platform |
extended chain and α-helix (1–56) | — | 76,80,98 | |
| Apc16 | 110 | 1 | scaffolding module TPR lobe | α-helix (52–110) | — | — | 8,16,26 |
| Cdc20/ Cdh1 |
499/496 | 1 | degron recognition module | NTD (73–135/42–163) | WD40 domain (168–471/172–473) | IR tail (492–499/483–496) | — |
| UbcH10 | 179 | 1 | catalytic module | UBC domain (30–179) | — | — | — |