FIG 9 .

VUF2274 is able to induce reactivation of HCMV from latently infected monocytes. CD14⁺ peripheral blood monocytes were isolated and infected at an MOI of 5 with RV1164, which has an IE2-YFP fluorescent tag. Three days postinfection, increasing concentrations of the US28 inhibitor VUF2274 were added to cells. (A) IE2-YFP-positive cells were counted by immunofluorescent microscopy at 72 h posttreatment. (B) Five days post-drug treatment, medium was removed from these cells and titrated onto HFFs. Three days postinfection, HFFs were fixed and stained for IE protein, and IE-positive cells were counted by immunofluorescence microscopy. (C) CD14⁺ peripheral blood monocytes were isolated and infected at an MOI of 5 with Titan-WT. Three days postinfection, increasing concentrations of the US28 inhibitor VUF2274 were added to cells. Five days post-drug treatment, UL32-GFP-positive cells were counted by fluorescence microscopy. (D) Cell survival of monocytes in the presence of VUF2274 for 4 days was measured using trypan blue exclusion staining. All data points show means of results from three replicates, and error bars show standard deviations. Data were subjected to analysis of variance (ANOVA) followed by Tukey’s post hoc test. *, P = 0.05 (statistically significant result).