Figure 1.

Twa1 is significantly upregulated in human CRC tissues. (A) Bioinformatics analysis of Twa1 expression in human CRC tissues and nontumor tissues from the Hong CRC microarray data set (GSE9348) available in the Oncomine database. Some genes highly significantly associated with CRC are listed. CXCL1, chemokine (C-X-C motif) ligand 1; DNMT1, DNA (cytosine-5-)-methyltransferase 1; FOXQ1, fork-head box Q1; LGR5, leucine-rich repeat containing G protein-coupled receptor 5. (B, C) Relative expression of Twa1 mRNA in human CRC tissues and their matched nontumor tissues in the CRC RNA-seq data set obtained from the TCGA database (B) and in quantitative RT-PCR (qRT-PCR) analysis of our own clinical samples (C). Each point represents log₂ transformed Twa1 expression relative to either TBP (TATA binding protein) or small nuclear RNA U6 expression in a single sample. Black horizontal bars show the median ± SD. P < 0.0001, Student's t-test. (D, E) Western analysis of Twa1 protein levels in CRC tissues and their matched nontumor tissues. The densities of Twa1 bands are quantified by Image J software (NIH) and normalized to actin, a loading control. Data are presented as log₂ value of Twa1 (T/N). P < 0.01, Student's t-test.