Figure 3.

The interface between TIN2_TRFH and TPP1_TBM. (A) Details of hydrophobic interactions around H1 and L₁₂ of TPP1. The interaction residues are presented as ball-and-stick models. TPP1 residues are colored in yellow and TIN2 residues are colored in cyan. (B) Details of hydrophobic contacts between H2 of TPP1 and TIN2. (C, D) Effects of mutations in the TPP1_TBM (C) and TIN2_TRFH (D) domains on the interaction between TIN2 and TPP1 analyzed in a yeast two-hybrid assay. Data are the average of three independent β-galactosidase measurements.