Table 1.
Distribution of proteins identified by comparative quantitative proteomic (MS) in different subtypes of EVs isolated from human monocyte-derived DCs [26]. Validation by western blot (WB) was performed for most of them on large (2 K pellets), medium (10 K pellets) and small EVs (100 K pellets) isolated from DCs and the listed established human cell lines, or mouse bone marrow-derived DCs (BMDCs). Information on antibodies used for WB is provided in Kowal et al. [26]. Proteins highlighted in bold are those identified by immunoisolation as probably specific to MVE-derived exosomes, bearing simultaneously CD63 and CD9 or CD81.
| protein name/gene/UNIPROT ID | vesicle type | detection method | cell type |
|---|---|---|---|
| actin cytoplasmic/ACTB, ACTG1/ACTB, ACTG | all EVs | MS, WB | DCs |
| annexin II or A2/ANXA2/ANXA2 | all EVs | MS, WB | DCs |
| flotillin-1/FLOT1/FLOT1 | all EVs | MS, WB | DCs |
| HSC70/HSPA8/HSPC7 | all EVs | MS, WB | DCs, MDA-MB-231, IGROV, OV2008, SHIN, HeLa, HEK, RPE1, mouse BMDCs |
| HLA (MHC) class I/HLA-A, HLA-B, HLA-C/1A*, 1B*, 1C* | all EVs | MS, WB | DCs |
| HLA (MHC) class II/HLA-DR**, HLA-DP**, HLA-DQ**/2B**, DP*, DQ*, DR* | all EVs | MS, WB | DCs, mouse BMDCs |
| alpha-actinin-4/ACTN4/ACTN4 | large EVs | MS, WB | DCs, MDA-MB-231, IGROV, OV2008, SHIN, mouse BMDCs |
| GP96 = endoplasmin/HSP90B1/ENPL | large EVs | MS, WB | DCs, MDA-MB-231, IGROV, OV2008, SHIN, mouse BMDCs |
| lysosome-associated membrane glycoprotein 2/LAMP2/LAMP2 | large EVs | MS, WB | DCs |
| mitofilin/IMMT /IMMT, MIC60 | large EVs | MS, WB | DCs |
| annexin XI or A11/ANXA11/ANX11 | small EVs | MS, WB | DCs |
| disintegrin and metalloproteinase domain-containing protein 10/ADAM10/ADA10 | small EVs | MS, WB | DCs, MDA-MB-231, IGROV, OV2008, SHIN, HEK, RPE1 |
| EH domain-containing protein 4/EHD4/EHD4 | small EVs | MS, WB | DCs |
| fibronectin/FN1/FINC | dense small EVs | MS | DCs |
| CD9 (tetraspanin)/CD9/CD9 | abundant in small EVs but also present in large EVs | MS, WB | DCs, MDA-MB-231, IGROV, OV2008, SHIN, HeLa, HEK, RPE1, mouse BMDCs |
| CD63 (tetraspanin)/CD63/CD63 | in small EVs: specific for exosomes. Also present in large EVs | MS, WB | DCs, MDA-MB-231, OV2008, HeLa, HEK, RPE1, mouse BMDCs |
| CD81 (tetraspanin)/CD81/CD81 | small EVs | MS, WB | DCs, MDA-MB-231, IGROV, OV2008, SHIN, HeLa, HEK, RPE1 |
| TSG101/TSG101/TS101 | small EVs | MS, WB | DCs, mouse BMDCs |
| syntenin-1/SDCBP/SDCB1 | small EVs | MS, WB | DCs, MDA-MB-231, IGROV, OV2008, SHIN, HeLa, HEK, RPE1, mouse BMDCs |