Table 4.
Relative abundances of orcokinin peptides from methyl esterified tissue extracts from a single SG or paired CoGs from Homarus americanus or paired CoGs from Procambarus clarkii analyzed using MALDI-FTMS.
| Homarus americanus Relative peptide abundance | Procambarus clarkii Relative peptide abundance | ||||
|---|---|---|---|---|---|
| Sequence, Peptide | Sinus gland (SG)a |
Commissural ganglia (COG)a |
Predictedb | Commissural ganglia (COG)a |
Predictedc |
| NFDEIDRSGFGFH [His13]-orcokinin | 15.1 (1.4) |
16.2 (1.7) |
28.6 | — | — |
| NFDEIDRSGFGFN [Asn13]-orcokinin | 45.5 (0.9) |
50.3 (3.5) |
42.9 | 67.7 (2.1) |
60/66.7 |
| NFDEIDRSGFGFV [Val13]-orcokinin | 21.1 (1.6) |
20.9 (1.2) |
28.6 | 13.0 (2.7) |
20/16.7 |
| NFDEIDRTGFGFH [Thr8-His13]-orcokinin | — | — | — | 2.4 (0.4) |
10/8.3 |
| NFDEIDRSGFGFV [Ala13]-orcokinin | — | — | — | 6.7 (0.4) |
10/8.3 |
| Truncated Orcokinins | |||||
| NFDEIDRSGFGF Orc [1-12] | 15.6 (2.4) |
10.7 (2.8) |
— | 5.3 (0.9) |
— |
| NFDEIDRSGFG Orc [1-11] | 2.2 (1.7) |
1.9 (1.0) |
— | 3.6 (0.4) |
— |
| NFDEIDRSGF Orc [1-10] | 0.5 (0.2) |
— | — | 0.2 (0.3) |
— |
| NFDEIDRTGFGF [Thr8-Xxx13] | — | — | — | 1.2 (0.1) |
— |
a
Single sinus glands or paired commissural ganglia tissues were subjected to solvent extraction followed by methyl esterification. The methyl esterified extracts were analyzed using DHB as the matrix.
b
Relative abundance predicted based upon the number of copies of the orcokinin peptide in the precursor proteins.