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Primer names, sequences, and annealing temperatures used in PCR amplification of the NAT2 gene.
| Primer | Primer sequence (5ʹ–3ʹ) | AT (°C) | Region | Size (bp) |
|---|---|---|---|---|
|
NAT2 1st-F |
GTCACACGAGGAAATCAAATGC | 57 | Exon 2 | 540 |
|
NAT2 1st-R |
TCCTCTCTCTTCTGTCAAGCAG | |||
|
NAT2 2nd-F |
GAATTACATTGTCGATGCTGG | 55 | Exon 2 | 610 |
|
NAT2 2nd-R |
TGAGGGTAGAGAGGATATCTGA |
F, forward primer; R, reverse primer; AT, annealing temperature.
