Figure 9.

EDA9 interacts with both SPF1 and SUMO1. EDA9 proteins localize in the chloroplast but interact with both SUMO1 and SPF1 proteins at specific sites in the cytoplasm and adjacent to the nuclei. GFP, EDA9:GFP; YFP, interaction between EDA9 (marked with cYFP at the C terminus) and SPF1 (marked with nYFP at the N terminus) or SUMO1 (marked with nYFP at the C terminus just before the double Gly residues); RFP, nuclear marker AHL22-mRFP (Xiao et al., 2009); Chl, chlorophyll; Bright, white light; Merge, merged images. Bars = 10 μm.