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. 2017 Oct 31;175(4):1661–1668. doi: 10.1104/pp.17.00164

Figure 3.

Figure 3.

The PHO1-overexpressing lines are insensitive to exogenous ABA during seed germination. A, qRT-PCR assay of PHO1 expression. The PHO1-overexpressing lines (PHO1pro:PHO1-12 and PHO1pro:PHO1-15) and wild-type plants were germinated and grown on MS medium for 1.5 d and then harvested for RNA extraction. Data are shown as mean ± se (n = 3). B, Phenotypic comparison. Imbibed seeds were germinated and grown on MS medium with 0 or 0.5 µm ABA for 10 d. C, Germination rate measurement. Imbibed seeds were transferred to MS medium with 0, 0.5, or 1 µm ABA for 3 d, and then the seed-germination rates were calculated. Data are shown as mean ± se (n = 3). Asterisks indicate significant differences compared with corresponding wild-type plants (#): *P < 0.05 and **P < 0.01.