Figure 4.
Progeny from drought-exposed lineages show enhanced seed dormancy. A, Independent dormancy assays performed on seeds from P0 (n = 6; more than 25 seeds per plate) and P1 (n = 9; more than 25 seeds per plate) progeny of G4 plants (P0 and P1 seeds, respectively). Points denote mean proportions of seeds germinated; error bars denote se. HR denotes the calculated hazard ratio from a fitted Cox proportional hazards model, representing the likelihood of germination between groups (HRD = drought versus unstressed lineage). B, Dehydration assay performed on detached rosettes of P1 progeny of G4 (n = 12) and G5 (n = 11) plants (independent experiments). A second-order polynomial regression, with a 95% confidence interval (shading), was performed to determine the coefficient for the lineage predictor term (α2). R2C denotes the conditional R2 calculated to assess model fit. C, Survival under the terminal drought experiment on transgenerational descendants (unstressed, n = 44; drought, n = 51). Bars denote means, and error bars denote se across two independent experiments.