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. 2017 Oct 6;175(4):1893–1912. doi: 10.1104/pp.17.00744

Table IV. Photosynthetic parameters, and corresponding equations, to nondestructively assay the impact of the drought stress utilized in this study.

Parameter Equation Interpretation
Fv FmFo Variable fluorescence, the ability of PSII to perform photochemistry
Fv Fm′ – Fo
Fq Fm′ – Ft Photochemical quenching of fluorescence by open PSII centers
Fv/Fm (FmFo)/Fm Maximum quantum efficiency of PSII
Fv′/Fm (Fm′ – Fo′)/Fm Estimate of the maximum quantum efficiency of PSII under actinic light
ΦPSII Fq′/Fm PSII quantum efficiency: the proportion of light absorbed by chlorophyll used for PSII photochemistry
qP Fq′/Fv Coefficient of photochemical quenching: relates PSII maximum efficiency to PSII operating efficiency
qL (Fq′/Fv′)/(Fo′/Ft′) Estimates the fraction of open PSII centers
NPQ (Fm/Fm′) – 1 Nonphotochemical quenching constant: estimates the rate constant for heat loss from PSII
Rfd (Fp/Ft′) – 1 Fluorescence decline ratio calculated using steady-state fluorescence: correlates with CO2 fixation rate, with Rfd > 3 indicative of highly efficient PSII and Rfd < 1 reflecting negligible net CO2 gain; this correlation allows this to be used as a vitality index; here, Fp is taken during the initial phase of the Kautsky effect, where the peak fluorescence is induced by actinic light