Skip to main content
. 2017 Oct 25;175(4):1839–1852. doi: 10.1104/pp.17.01152

Figure 4.

Figure 4.

A portion of MKP1 is constitutively degraded by the proteasome in naïve plants. A, Fourteen-day-old transgenic seedlings expressing myc-MKP1 were treated with or without 40 µm MG132 for the times indicated. Protein extracts from treated seedlings were separated by 8% mini-format (8.3 × 7.3 cm) SDS-PAGE and immunoblotted with anti-myc antibody to detect the myc-MKP1 protein (top) or anti-MPK6 antibody used as a loading control (bottom). B, Fourteen-day-old transgenic seedlings were pretreated with or without 40 µm MG132 for 40 min and then treated with 1 µm elf26 for 20 min. Protein extracts from treated seedlings were separated by 8% mini-format (8.3 × 7.3 cm) SDS-PAGE and immunoblotted with anti-myc antibody to detect the myc-MKP1 protein (top), anti-phospho-p42/44 antibody to detect phosphorylated MAPKs (middle), or anti-MPK6 antibody as a loading control (bottom). This experiment was performed three times with similar results to those shown.