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. 2017 Oct 25;175(4):1853–1863. doi: 10.1104/pp.17.01223

Figure 2.

Figure 2.

Functional assessment of PsKPP4, PsFUZ7, and PsKPP6 in the wheat-Pst interaction by virus-induced gene silencing. A, Phenotypes of fourth leaves inoculated with CYR32 at 14 dpi. Plants were preinoculated with FES buffer (mock), BSMV:TaPDS, BSMV:γ, BSMV:PsKPP4-1as, BSMV:PsKPP4-2as, BSMV:PsFUZ7-1as, BSMV:PsFUZ7-2as, BSMV:PsKPP6-1as, or BSMV:PsKPP6-2as, respectively. B, Relative transcript levels of PsKPP4, PsFUZ7, and PsKPP6 in knockdown plants inoculated with CYR32 at 2 and 7 dpi. Wheat leaves inoculated with BSMV:γ and sampled after inoculation with CYR32 were used as controls. Data were normalized to the transcript level of PsEF-1. Asterisks indicate P < 0.05; double asterisks indicate P < 0.01. C, Quantification of uredial density in silenced plants 14 dpi with CYR32. Differences were assessed using Student’s t tests. Values represent the means ± se of three independent samples. Single asterisks indicate P < 0.05; double asterisks indicate P < 0.01.