Figure 6.

Microscopy visualization of the in host-induced gene-silencing effect targeting PsFUZ7 on colonization of wheat leaf tissue by Pst. A, Cytological observation of rust interaction with wheat by epifluorescence microscopy. Leaves inoculated with CYR32 were sampled at 10 dpi. Bars = 50 μm. B, Infection area per infection site is reduced in the transgenic lines L65 and L91 infected with CYR32 compared with control at 10 dpi. C, The formation of haustoria in lines L65 and L91 is inhibited after inoculation with CYR32 compared with control at 10 dpi. D, The necrotic area of plants from lines L65 and L91 is increased compared with control at 10 dpi with CYR32. E, Cytological observations of Pst CYR32 and wheat by transmission electron microscopy at 10 dpi. Left bars = 2 μm; right bars = 500 nm. Differences in D and E were assessed using Student’s t tests. Values represent the means ± se of three independent samples. Double asterisks indicate P < 0.01. Ch, Chloroplast; CK, control; Cy, cytoplasm; H, haustorium; HC, host cell; HMC, haustorial mother cell; IH, infection hypha; NHC, necrotic host cell; Org, organelle; RN, rust nucleus; SV, substomatal vesicle.