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Effect of oxysterols on osteogenic-related protein levels. Protein levels of OCN, OSX, and RUNX2 determined by (a–c) immunofluorescence staining and (d) western blotting (OCN 5.5 kDa, OSX 45 kDa, RUNX2 55 kDa). Nuclei stained with DAPI (blue). Representative result from three independent experiments (scale bar, 100 μm). DAPI 4′,6-diamidino-2-phenylindole, OCN osteocalcin, OSX osterix, RUNX2 Runt-related transcription factor 2
