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. 2017 Dec 5;17:820. doi: 10.1186/s12885-017-3809-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Immunohistochemistry and manual scoring analysis of Australian Breast Cancer Tissue Bank patient samples. Immunohistochemistry was performed on formalin-fixed paraffin embedded breast cancer patient tissue samples (n = 236) obtained from the Australian Breast Cancer Tissue Bank (ABCTB) using antibodies to detect and measure relative levels of IGF1R, p-IGF1R and SphK1. The intensity of immunostaining was assessed by manual scoring according to standard guidelines as follows: 0 = no staining, 1 = weak staining, 2 = moderate staining and 3 = strong staining for IGF1R, p-IGF1R and SphK1