Table 3.
Trans-splicing sites in three Culicidae
| An. stephensi | ||||||||
| donor | acceptor | |||||||
| contig | position | strand | sequence around splice site* | Contig | position | strand | sequence around splice site* | |
| Tm1.1 | stI-e1 | 8284604 | − | ATCAAGAAGGATAATCATAACTGCA|GT | stI-e2 | 29650741 | − | AC|TGTTGGTTGGGAGGGTGAACAACGG |
| Tm1.2 | stI-e3 | 29650494 | − | CGCAATGCTGCTGAAGCGTGTTGCG|GT | stI-e2 | 49700929 | + | AG|GAGTTGCAAAATCAGGTTGATTTTC |
| Tm2 | stI-e1 | 10533884 | − | ATCTGTTCGATGATGATCGAAAGTT|GT | stI-e2 | 13176261 | + | AG|TATCGCACACGGTACAAGATTTGGT |
| Tm3 | stI-e4 | 31171572 | + | CATCACTACTCCTGCCATCTGTGTC|GT | stI-e1 | 4936658 | − | AG|GGCGTATTTATCTTCAACATCGTGC |
| Tm4 | stI-e4 | 31171572 | + | CATCACTACTCCTGCCATCTGTGTC|GT | stI-e1 | 4948239 | + | AG|GGCGTATTTATCTTCAACATCGTGC |
| Tm5 | stI-e1 | 14042259 | + | AAAGCTGAAAGATGTCGTTGATCAG|GT | stI-e2 | 31153557 | − | AG|CAAAGTTCCTTCGCCTGCTGGCTAC |
| An. gambiae | ||||||||
| donor | acceptor | |||||||
| contig | position | strand | sequence around splice site* | Contig | position | strand | sequence around splice site* | |
| Tm1.1 | X | 14803808 | − | ATCAAGAAGGACAATCACAACTGCA|GT | 2L | 40170764 | + | AG|TTGAGCGGCGTCGTCGATTCAACAT |
| Tm1.2 | 2L | 40171011 | + | CGCAATGCTGCAGAAGCGTGTCGCG|GT | 2R | 56727316 | + | AG|GAGTTGCAAAACCAAGTTGACTTTC |
| Tm2 | X | 4334405 | − | ATCTGCTCGATGATGATCGAAAGTT|GT | 2R | 13216641 | − | AG|TATCGCACACGGTACAGGATTTGGT |
| Tm3 | 3R | 42647718 | + | CATCACCACTCCTGCCATCTGTGTC|GT | X | 8704053 | − | AG|GGCGTATTCATCTTCAACATCGTGC |
| Tm4 | 3R | 42647718 | + | CATCACCACTCCTGCCATCTGTGTC|GT | X | 8717291 | + | AG|GGCGTATTCATCTTCAACATCGTGC |
| Tm5 | X | 1025131 | + | AAAGCTGAAAGATGTCGTTGATCAG|GT | 2R | 27239887 | + | AG|CAAAGTTCCTTCGCCTGCTGGCTAC |
| An. gambiae | ||||||||
| donor | acceptor | |||||||
| contig | position | strand | sequence around splice site* | Contig | position | strand | sequence around splice site* | |
| Tm1.1 | supercont1.30 | 2525719 | + | ATCAAGAAGGATAACCATAACTGCA|GT | supercont1.497 | 69990 | − | AG|TCGAGAGGCGTCGTCGTTTCAATAT |
| supercont1.322 | 325659 | − | ATCAAGAAGGATAACCATAACTGCA|GT | supercont1.497 | 69990 | − | AG|TCGAGAGGCGTCGTCGTTTCAATAT | |
| Tm1.2 | supercont1.497 | 69743 | − | CATCATTCTGCAGCACAAACTGGCG|GT | supercont1.541 | 339301 | − | AG|GAAATGCAAAAGCAGCTCGACTATT |
| Tm2 | supercont1.75 | 2116294 | − | GTTTGCTCGATGATGATCGAAAGTT|GT | supercont1.187 | 287309 | − | AG|TATCCCACACCGTTCAGGATTTGGT |
| Tm3/Tm4 | supercont1.496 | 104246 | − | TATCACGACTCCGACGATCTGTGTT|GT | supercont1.715 | 64386 | + | AG|GGCGTATTCATCTTCAACCTGGTGC |
| Tm5 | supercont1.54 | 128027 | − | GAAGCTGAAGGACGTAGTCGATCAG|GT | supercont1.179 | 490627 | − | AG|CAAAGTTCCTTTGCCTGCTGGTTAC |
*
splice junctions are indicated as “|”. 25 bp upstream of donor site and 25 bp downstream of acceptor site are shown.