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. 2017 Dec 6;8:275. doi: 10.1186/s13287-017-0716-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Difference in osteogenesis ability between ATSCs and BMSCs in vitro. a–d Total RNA extracted from ATSCs and BMSCs or cells subjected to OIM for 7 days. Relative expression levels of ALP, Runx2, OPN, and OCN checked by qRT-PCR. β-actin as an internal control. Data expressed as mean ± SD (n = 3). *p < 0.05, compared with MSCs in OIM; #p < 0.05, compared with MSCs in α-MEM. e, f Alizarin Red S staining of calcium deposits formed by ATSCs and BMSCs. ATSCs and BMSCs cultured in OIM for 14 days, then cells fixed and stained with Alizarin Red S. ALP: alkaline phosphatase, ATSC: adipose tissue-derived MSC, BMSC: bone marrow-derived MSC, MEM: minimum essential medium, MSC: mesenchymal stem cell, OCN: Osteocalcin, OIM: osteogenic induction medium, OPN: Osteopontin, Runx2: runt-related transcription factor 2