Table 1.

Primer sequences forward and reverse primer sequences for real time qPCR and in situ hybridisation analysis of Eph/ephrinA mRNA expression in visual structures of P0 Ten-m3 KO mouse

Gene name	NCBI mRNA accession #	Primer sequences 5′–3′
Glyceraldehyde-3-phosphate dehydrogenase (Gapdh)	NM_001001303	F_AACTTTGGCATTGTGGAAGG R_ACACATTGGGGGTAGGAACA
EphrinA2 (Efna2)	NM_007909	F_AGCATCAACGACTACCTGGA R_AAAAGGGGGTGAAGAGTTGG
EphrinA5 (Efna5)	NM_010109.3	F_AGCAACCCCAGATTCCAGAGG R_TTTTCCGAGAACTTCAGCG
Eph receptorA5 (Epha5)	NM_007937.3	F_TGCTATTCGGCACCTCTAAAGG R_GGGGGCATAGTTCTCATCAA
Eph receptor A6 (Epha6)	NM_007938.2	F_CTCCAGCCTTCCCTTCACC R_TATCTATCATCAAACTCCACACA
Eph receptor A7 (Epha7)	NM_010141.3	F_GCTGTAAATGGAGTTTCGGAC R_GTGTGGCAACATCAAGCCTA
Zinc finger transcription factor 2 (Zic2)	NM_009574.3	F_AATGGCTTATTGGCTTATTGG R_ACTTTGGCACGGCTCATATT
Ephrin ligand B1 (EfnB1)	NM_010110.4	F_ACCCGAGCAGTTGACTACCA R_ATGATGAGCAGGAAGATGACAC
Eph Receptor B1 (EphB1)	NM_173447.3	F_TGGCTATGGCAAGTTCAGTG R_CAATGTAGATCTTCATCCCT

Gapdh was used as a reference gene in real time qPCR assays