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. 2017 Dec 6;36:175. doi: 10.1186/s13046-017-0646-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — FOXA1 regulates the expression of PIK3R1. Based on the FOXA1 conserved motif (b), we explored FOXA1 target genes in Cistrome dataset_browser and ChIPBase_v2.0 datasets (a) combined with GO enrichment analysis (c, d), and we selected our target gene of interest, PIK3R1. qPCR revealed that downregulation of FOXA1 decreased the PIK3R1 mRNA level, whereas plasmid overexpression of FOXA1 increased PIK3R1 expression (e, f). In HepG2 (g, h) and Hep3B (i, j) cells, decreased FOXA1 expression promoted PI3Kp85 expression, thereby increasing PI3K/AKT activity, whereas increased FOXA1 expression suppressed PI3K/AKT activity through PI3Kp85 suppression. GAPDH was used as the loading control