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. 2017 Dec 6;10:689. doi: 10.1186/s13104-017-3005-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Comparison of Cq values from RT and No-RT samples. Three of the seven primer/probe sets detected signals from No-RT controls (n = 8–10) in qPCR including Actb, Gapdh and Ywhaz. When the Cq values of No-RT controls were compared to those of RT samples (RNA samples went through reverse transcription step), significant larger values of No-RT controls was observed for Actb and Ywhaz, but not for Gapdh