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. 2017 Dec 6;10:689. doi: 10.1186/s13104-017-3005-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Evaluation of reference gene expression. a Gene expression by RT-qPCR. Average Cq values from duplicate qPCR reactions of each aLN (n = 30) and iLN sample (n = 29) were used for data analysis. The box indicates 25–75% with median shown in the box and the whiskers represent the minimum to maximum range. b Scores determined by RefFinder including the comprehensive stability, geNorm, NormFinder, BestKeeper and comparative delta-Ct method are shown for 7 reference genes. c Scores determined by RefFinder including the comprehensive stability, geNorm, NormFinder, BestKeeper and comparative delta-Ct method are shown for 4 reference genes